帳號：
密碼：
奈米鑽石醫學&保養品化妝品
Facebook
Twitter
Plurk
列印本頁
商品詳細說明
–hand cleaning gel
–Environment sterilization agent
–Mouth gargle agent & tooth paste
–Condom for protect HIV, HPV,HSV
–Water filtration membrane
–Air conditioner filter (prevantdisease outbreak)
About swine flu H1N1:
nanodiamond
&
Influenza Virus
sterilization mechanism?
Influenza virus
Possible mechanisms of ND with different functional groups
About swine flu H1N1:
nanodiamond
&
Influenza Virus
sterilization mechanism?
Influenza virus
Possible mechanisms of ND with different functional groups
•Protein Capsid destruction
(as 台大抗煞一號,電性相斥崩解)
•8 pieces (-)RNA genome:
–11 proteins: hemagglutinin(HA), neuraminidase(NA), nucleoprotein (NP), M1, M2, NS1, NS2(NEP), PA, PB1, PB1-F2 and PB2
–破壞此8段RNA中重要基因結構(人體無negative RNA?)
–攜帶核苷酸類似物插入virus RNA製造複製過程停滯
–破壞其RNA-dependence RNA polymerase(人體內無此enzyme)
•更強之M2 channel或 NA 抑制劑
•Lipid envelope destruction?(親油性?)
Mapping the sequence mutations of
the 2009 H1N1 influenza A virus
neuraminidase relative to
drug and antibody binding sites
Biology Direct 2009, 4:18
Cysteine C49—anchor?? Intermolecular disulfide bond??
.jpg)
ND application for H1N1
Mapping the sequence mutations of
the 2009 H1N1 influenza A virus
neuraminidase relative to
drug and antibody binding sites
Biology Direct 2009, 4:18
•sequence variations of the “2009 H1N1” influenza A virus strain neuraminidase
•Homology-based protein 3D structure modeling
reveals that the novel mutations
•3 currently used neuraminidase inhibitors:
oseltamivir(Tamiflu®), zanamivir(Relenza®) and peramivir
•the antigenic regionsof the neuramidaserelevant for vaccine development, serological typing andpassive antibodytreatment
Cysteine C49—anchor?? Intermolecular disulfide bond??
ND application for H1N1
•neuraminidase destruction (protein level)
–Cysteine 49? Destruct disulfurbond or lipid anchor
–Destruct N104 & N146 for protein polymerization
•Enhance the NA inhibitor efficiency
ND application for immune
ND application for immune
•Vaccine:
–Traditional: carrier of antigen into human body
–Gene vaccine: carry the antigenic genes into human genomes
•Artificial antibody:
–epitope peptide chain synthesis
–Carry genes into B cells?
ND application for medical examination
ND application for medical examination
•Allergen test
–測血清中allergen-specific IgEantibodies,目前僅有skin test & 健保給付35種常見過敏原檢查
–未知allergen,具有allergic shock之危險!
–以nanodiamond二維矩陣製成千至萬種之過敏原檢驗盤, 再以血清反應觀察IgE結合強度,增強藥物過敏篩檢效率
ND application for medical examination
Using Detonation Nanodiamond
for the Specific
Capture of Glycoproteins
Anal. Chem. 2008, 80, 4659–4665
ND application for medical examination
•Viral serology test
–病毒檢測方法僅限數種
–需在活細胞中培養,如雞胚
–空窗期之考量,無抗體產生,故呈陰性
–
–Nanodiamond可辨認病毒外殼glycoprotein—
(ie.病毒靠岸之船錨)Using Detonation Nanodiamond
for the Specific
Capture of Glycoproteins
Anal. Chem. 2008, 80, 4659–4665
•the functionalization of detonation nanodiamond(ND) with aminophenylboronicacid (APBA)
for the purpose of targeting the selective capture of glycoproteins from unfractionated protein mixtures
•A loading capacity of ∼350 mg of glycoprotein of ND could be attained on ND
•functionalized ND as a high-efficiency extraction and analysis platform for proteomics research
Nanodiamond:
Biocompatiblity
&
non-cytotoxic
For implant coating, local drug delivery and in vivo usage?
Protein-mediated assembly of nanodiamondhydrogels into a biocompatible and biofunctionalmultilayer nanofilm ACS Nano. 2008 Feb;2(2):203-12
via the layer-by-layer deposition technique
(1)cellular gene expression examination by real-time polymerase chain reaction
(2)MTT and DNA fragmentation assays
Nanodiamond:
Biocompatiblity
&
non-cytotoxic
For implant coating, local drug delivery and in vivo usage?
Protein-mediated assembly of nanodiamondhydrogels into a biocompatible and biofunctionalmultilayer nanofilm ACS Nano. 2008 Feb;2(2):203-12
•diameter of 2-8 nm
•assembled into a closely packed ND multilayer nanofilm
with positively charged poly-L-lysinevia the layer-by-layer deposition technique
•The innate biocompatibility of the NDs:
(free-floating and thin-film forms)(1)cellular gene expression examination by real-time polymerase chain reaction
(2)MTT and DNA fragmentation assays
•Knockdown of lipopolysaccharide-mediated inflammation
Are Diamond Nanoparticles Cytotoxic?
J. Phys. Chem. B, Vol. 111, No. 1, 2007
Are Diamond Nanoparticles Cytotoxic?
J. Phys. Chem. B, Vol. 111, No. 1, 2007
•nanodiamondssize from 2 to 10 nm
•Assays of cell viability
(1)mitochondrial function(MTT) (2)luminescent ATP production
•not produce significant reactive oxygen species
•Cells can grow on nanodiamond-coated substrates without morphological changes
ND-raw ND-COOH CB(-) CdO(+)
ND-raw CB CdO
NO Mitochondrial membrane & cytoskeleton change
ND-raw ND-COOH CB(-) CdO(+)
ND-raw CB CdO
NO Mitochondrial membrane & cytoskeleton change
(1)internalized nanodiamondsafter exposure to 25 íg/mLND-COOH for 24 h.
(2)cells grown on a ND-COOH coated collagen substrate after 96 h
ROS production test
not produce significant reactive oxygen species
ROS production test
not produce significant reactive oxygen species
•Implant coating
–Artificial joint(骨髓炎之預防,加入ND合併微小包覆體抗生素釋出)
•無排斥性植入式晶片
–失智老人,精神分裂患者,寵物
–Biosensor: 血糖,血脂肪,血壓值
•長期投與藥物釋出系統
–避孕荷爾蒙類藥物
–癌末病人止痛如fentanyl
II.Biochemicalmolecule absorption & excretion?
Health food or pharmacy
防曬,除皺,美白?
Bipolar Fractional Radiofrequency
Treatment Induces
Neoelastogenesisand Neocollagenesis
Lasers in Surgery and Medicine 41:1–9 (2009)
II.Biochemicalmolecule absorption & excretion?
Health food or pharmacy
•Lipid
–減重
•目前僅有Xenical(Orlistat)是抑制胰脂肪酶,減緩30%腸道脂肪吸收其餘皆為抑制食慾或促交感神經藥物
–ND於腸道中吸附攝食之油脂(如何定量?)
–目前有1064nm Nd-YAG Laser於熔解皮下脂肪之應用,但仍需傷口使脂肪抽吸出體外
•ND 可進入細胞層次,或可藉由ultrasound加速其深入帶出脂肪?
•ND 可進入細胞層次,或可藉由ultrasound加速其深入帶出脂肪?
•Lipid
–Fatty acid
•增強攜帶多元unsatuatedFA 被細胞利用能力
,如魚油
–Cholesterol
•降低血清中低密度脂蛋白(LDL)濃度,預防心血管疾病
•已存在之血管璧脂肪條塊或粥狀硬化中,使fat之清除(血管清道夫),延緩冠狀動脈硬化心肌梗塞
•Diabetes
–Type I : insulin gene therapy carrier
–Type II:
•Enhance blood sugar into cell ,reduce blood sugar conc.
•Absorption and excretion excess insulin
•
•排毒??(諾得膠囊—active charcoal only)
–腎: creatinine,bloodurea nitrogen,uricacid
–肝:ammonia, bilirubin
Keloid or hypertrophic scar
Others
Keloid or hypertrophic scar
•Type I , III collagen增生
•Surgery: 50%復發
•注射類固醇:3~6months復發
•Laser, cryotherapy:傷害大
•
•ND可攜帶collagenase或新研發中之
alfa-interferon, 5-FU, bleomycin進入疤痕組織Others
•抽油煙機濾網
•生命事業
–胎毛,結婚,葬儀紀念
•傳熱性佳應用於鍋具,烹飪爐具烘烤用途
III.Aesthetic& cosmetics
皮膚厚度:平均0.2mm
最外角質層:à黯沉淨白
最內基底層:細胞分裂,決定皮膚外觀à修復
真皮: 合成膠原蛋白&彈性蛋白à回春除皺
細胞間隙:natural moisturingfactor (主含玻尿酸)à保濕
毛孔粗大à過度出油 or保濕不足
黑色素合成
III.Aesthetic& cosmetics
皮膚厚度:平均0.2mm
最外角質層:à黯沉淨白
最內基底層:細胞分裂,決定皮膚外觀à修復
真皮: 合成膠原蛋白&彈性蛋白à回春除皺
細胞間隙:natural moisturingfactor (主含玻尿酸)à保濕
毛孔粗大à過度出油 or保濕不足
黑色素合成
防曬,除皺,美白?
•UVA1,2(98%)320~400nmàPA係數: 曬黑
•UVB(<2%)290~320nmàSPF :曬傷曬紅
•UVC(被O3吸收)
•物理性:
–Zinc oxide(收斂抗菌)àUVA&UVB
–TiO2:UVA&UVB
易阻塞毛孔
•化學性:
–Octylsalicylate(UVB)
–Parsol1789(UVA1 &UVB)
–MexorylSX(UVA & UVB)
–
•防曬+美白:nanodiamond鍵結美白成分深入黑色素細胞抑制tyrosinase,基底加入防曬成分
•防曬+美白+除皺緊實:
除上述美白成分,利用nanodiamond可深入毛孔及基底細胞與真皮層處,藉由熱能刺激膠原蛋白及彈性蛋白增生(同1064nm Nd-YAG Laser原理),達到除皺緊實以及縮小毛孔效果
•熱能之來源:
–由UV轉換而來?
美容儀器
美容儀器
•除皺紋老化
–如何達到與Laser同樣功效?
–利用放射腫瘤科治療前的simulation原理,做出適合個人臉型的構模,此為可塑性材料,接觸臉部含有nanodiamond
–或塗佈一層含有nanodiamond的介質於臉部,以光照或是其他能量使其傳熱於皮膚深部
Bipolar Fractional Radiofrequency
Treatment Induces
Neoelastogenesisand Neocollagenesis
Lasers in Surgery and Medicine 41:1–9 (2009)
•The FRF system delivered RF energy
directly within the dermis via 5 micro-needle electrode pairs
•Tissue temperature was held at 72C for 4 seconds
•marked induction of tropoelastin, fibrillin, procollagens1 and 3by 28 dayspost-treatment
臨床常見皮膚問題
臨床常見皮膚問題
•出油
–ND可吸附油脂
•毛孔粗大
–ND可深入毛孔,藉由熱能促進collagen增生縮小毛孔
•粉刺
–先以蛋白酵素去角質,ND再包覆吸附阻塞毛孔中的油脂,可利用靜電原理配合剝除面膜帶離
•青春痘
同上,但可再測試抗菌能力以及抗發炎附加功效,並加速帶走游離脂肪酸
•黑眼圈
–色素型:同美白原理
–血循不良型:同配合導熱性佳原理促進循環
–過敏鼻炎型:可作為鼻噴劑抑制局部發炎反應